RESUMO
Posttranslational modification of histone proteins is critical for memory formation. Recently, we showed that monoubiquitination of histone H2B at lysine 120 (H2Bub) is critical for memory formation in the hippocampus. However, the transcriptome controlled by H2Bub remains unknown. Here, we found that fear conditioning in male rats increased or decreased the expression of 86 genes in the hippocampus but, surprisingly, siRNA-mediated knockdown of the H2Bub ligase, Rnf20, abolished changes in all but one of these genes. These findings suggest that monoubiquitination of histone H2B is a crucial regulator of the transcriptome during memory formation.
Assuntos
Histonas , Memória , Transcriptoma , Ubiquitinação , Animais , Masculino , Ratos , Histonas/genética , Processamento de Proteína Pós-Traducional , Transcriptoma/genética , Ubiquitina-Proteína Ligases/genética , Ubiquitina-Proteína Ligases/metabolismoRESUMO
Over 25 years ago, a seminal paper demonstrated that the ubiquitin-proteasome system (UPS) was involved in activity-dependent synaptic plasticity. Interest in this topic began to expand around 2008 following another seminal paper showing that UPS-mediated protein degradation controlled the "destabilization" of memories following retrieval, though we remained with only a basic understanding of how the UPS regulated activity- and learning-dependent synaptic plasticity. However, over the last 10 years there has been an explosion of papers on this topic that has significantly changed our understanding of how ubiquitin-proteasome signaling regulates synaptic plasticity and memory formation. Importantly, we now know that the UPS controls much more than protein degradation, is involved in plasticity underlying drugs of abuse and that there are significant sex differences in how ubiquitin-proteasome signaling is used for memory storage processes. Here, we aim to provide a critical 10-year update on the role of ubiquitin-proteasome signaling in synaptic plasticity and memory formation, including updated cellular models of how ubiquitin-proteasome activity could be regulating learning-dependent synaptic plasticity in the brain.
Assuntos
Complexo de Endopeptidases do Proteassoma , Ubiquitina , Feminino , Humanos , Masculino , Complexo de Endopeptidases do Proteassoma/metabolismo , Ubiquitina/metabolismo , Proteólise , Aprendizagem , Plasticidade Neuronal/fisiologiaRESUMO
This paper presents data collected from a 5.94 kWp grid connected photovoltaic (PV) plant implemented in hot semi-arid climate of Safi region, Morocco. The data include electrical power production and PV module temperature of three PV technologies: mono-crystalline (m-Si), poly-crystalline (p-Si), and amorphous (a-Si); they also include plane of array solar irradiance and ambient temperature. Solar irradiance was measured with calibrated reference cells, inverters provided the produced powers, and the temperatures were obtained by Pt100 probes. The data were measured each 5 min and were remotely accessible through internet. They were preprocessed to eliminate unrepresentative records and were used for the development of simple and accurate models for PV power forecasting [1]. These data are typical for hot semi-arid climate and may be reused for regional forecast of PV power as well as solar energy and PV module temperature predictions.